Naomi is completing her PhD at University of Technology Sydney
Proteomic characterisation of adipose stem cells
·Isolation of adipose stem cells from 10 human abdominal lipoaspirates.
·Expansion of cells using traditional media and xeno-free alternatives. Investigation of cryopreservation on adipose stem cells.
·Proteomic characterisation was achieved through analysing whole cell lysates, membrane bound fractions and extracellular vesicles on a Q Exactive Plus Orbitrap mass spectrometer.
·Examination of27 secreted cytokines with a Bioplex multiplex immunoassay.
Kynurenine pathway in adipose stem cells
·Collaboration I personally established with Dr Michael Lovelace and Prof Bruce Brew at St Vincent's Centre for Applied Medical Research.
·Adipose stem cells were treated with interferon gamma and the neurotoxin Quinolinic Acid for assay by proteomics and analysis of microscopy images.
·Kynurenine pathway metabolites were determined by Brew lab collaborators at Macquarie University by high-performance liquid chromatography and gas chromatography-mass spectrometry.
·Creation of digital media content for multiple sclerosis patients, utilisation of this digital media for education, and investigation of self-regulated learning.