Research

Naomi is completing her PhD at University of Technology Sydney

Proteomic characterisation of adipose stem cells

Isolation of adipose stem cells from 10 human abdominal lipoaspirates

Expansion of cells using traditional media and xeno-free alternatives. Investigation of cryopreservation on adipose stem cells

Proteomic characterisation was achieved through analysing whole cell lysates, membrane bound fractions and extracellular vesicles on a Q Exactive Plus Orbitrap mass spectrometer

Examination of 27 secreted cytokines with a Bioplex multiplex immunoassay


Kynurenine pathway in adipose stem cells

Collaboration with St Vincent's Centre for Applied Medical Research and Macquarie University

Adipose stem cells were treated with interferon gamma and the neurotoxin Quinolinic Acid for proteomic and microscopy analysis

Kynurenine pathway metabolites were determined by high-performance liquid chromatography and gas chromatography-mass spectrometry


Digital media

Creation of digital media content for multiple sclerosis patients, utilisation of this digital media for education, and investigation of self-regulated learning

Register your interest to participate here

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Publications

O’Rourke, M.B.; Town, S.E.L.; Dalla, P.V.; Bicknell, F.; Koh Belic, N.; Violi, J.P.; Steele, J.R.; Padula, M.P. What is Normalization? The Strategies Employed in Top-Down and Bottom-Up Proteome Analysis Workflows. Proteomes 2019, 7, 29.

 

Conferences

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2019

Human Proteome Organization World Congress

International Society for Stem Cell Research Annual Meeting
Los Angeles

Annual Lorne Proteomics Symposium

2018

International Society for Stem Cell Research Annual Meeting Melbourne

Annual Lorne Proteomics Symposium 

UTS Learning and Teaching Forum

Australian Science Communicators National Conference

UTS HDR Career Conference

2017

International Society for Stem Cell Research Annual Meeting Boston

Progress in Multiple Sclerosis Research Australia Conference

2016

Annual Lorne Proteomics Symposium

New South Wales Stem Cell Network Workshop

InSPiRE Workshop

2015

New Horizons Conference

 
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University of Technology Sydney
Facility leader: Dr Matthew Padula